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Human, mouse, and rat enteric glia express all C. difficile toxin receptors. Gene expression of C. difficile toxin receptors: Cspg4 , Tfpi, Lrp1, Pvrl3, Fzd1, Fzd3, Fzd7 , and Ldlr in human and mouse enteric glia, obtained through the Single‐Cell Portal (“the human and mouse enteric nervous system at single cell resolution”) . Gene expression of these receptors in rat enteric glia (PK060399egfr/ATCC) cells was measured by qPCR.

Journal: The FASEB Journal

Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

doi: 10.1096/fj.202600333R

Figure Lengend Snippet: Human, mouse, and rat enteric glia express all C. difficile toxin receptors. Gene expression of C. difficile toxin receptors: Cspg4 , Tfpi, Lrp1, Pvrl3, Fzd1, Fzd3, Fzd7 , and Ldlr in human and mouse enteric glia, obtained through the Single‐Cell Portal (“the human and mouse enteric nervous system at single cell resolution”) . Gene expression of these receptors in rat enteric glia (PK060399egfr/ATCC) cells was measured by qPCR.

Article Snippet: Immortalized rat enteric glial cell line PK060399egfr (ATCC CRL‐2690, VA, USA) was cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco) and supplemented with 10% bovine serum albumin (BSA), 1% antibiotics (100 μg/mL penicillin and 100 μg/mL streptomycin, Gibco), and 1 mM sodium pyruvate (Gibco) at 37°C in a humidified incubator under 5% CO 2 for no more than 24 passages.

Techniques: Gene Expression, Single Cell